Molecular Characterization of Staphylococcus aureus Isolates Obtained from Hemodialyzed Patients at the Hospital de Clínicas of Paraguay: A pilot study

  • Rubén Arturo Silvero Isidre, Universidad Nacional de Asunción, Paraguay.
  • Fátima Rodríguez Acosta Universidad Nacional de Asunción, Paraguay
  • César Rodrigo Cristaldo Vargas Universidad Nacional de Asunción, Paraguay
  • Genaro Américo Velázquez Romero Universidad Nacional de Asunción, Paraguay.
  • José Félix Plans Perrota Universidad Nacional de Asunción, Paraguay.
  • Rosa María Guillén Fretes Universidad Nacional de Asunción, Paraguay
Keywords: Staphylococcus aureus, Hemodialysis, Antibiotic resistance, bacterial typing


Background: Patients undergoing hemodialysis are susceptible to the nasal carriage of Staphylococcus aureus, increasing the risk of developing infections associated with higher morbidity and mortality. The objective of this study was to describe the frequency of S. aureus carriage in hemodialysis patients and to perform molecular analysis of isolates by applying multiple-locus variable analysis.

Methods: We conducted a descriptive cross sectional study with non-probabilistic sampling that included 28 hemodialysis patients attending the Nephrology Department of Hospital de Clínicas in Asunción, Paraguay. We obtained clinical data from medical records and interviews with patients. Nasal swabs were collected and analyzed by microbiological and molecular methods.

Results: The frequency of S. aureus carriage was 50% (14/28), 93% of which (13/14) were methicillin resistant, 57% (6/14) were gentamicin resistant and 36% (5/14) were resistant to more than 4 antibiotic classes. S. aureus carriers showed higher frequency of rhinitis (p=0.02 odds ratio [OR]=6.6 (1.2- 34.4)). Seven methicillin-resistant S. aureus isolates had been analyzed by multiple-locus variable analysis, two of them showed identical pattern bands.

Conclusion: We found a high frequency of methicillin-resistant Staphylococcus aureus colonization and the presence of two isolates with identical profile in the multiple-locus variable analysis indicating the possibility of transmission between patients.

Author Biography

Rubén Arturo Silvero Isidre,, Universidad Nacional de Asunción, Paraguay.

Artu¬ro Silvero Isidre is a fi¬nal-year medical student of a six-year program at the Universidad Nacional de Asunción, Paraguay. He is editor of a scientific journal called CIMEL and a member of a scientific so¬ciety of medical students (SOCIEM-UNA) in Paraguay


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How to Cite
Silvero Isidre, R. A., Rodríguez Acosta, F., Cristaldo Vargas, C. R., Velázquez Romero, G. A., Plans Perrota, J. F., & Guillén Fretes, R. M. (2017). Molecular Characterization of Staphylococcus aureus Isolates Obtained from Hemodialyzed Patients at the Hospital de Clínicas of Paraguay: A pilot study. International Journal of Medical Students, 5(1), 14-19.
Original Article